Phospho chk2 antibody
WebAnti-Chk2 (phospho T68) antibody See all Chk2 primary antibodies Description Rabbit polyclonal to Chk2 (phospho T68) Host species Rabbit Tested applications Suitable for: … WebJun 30, 2024 · Membranes were incubated in 5% nonfat milk for 45 min at room temperature and then incubated for 18 hours at 4°C with primary antibodies against the following molecules diluted in 5% nonfat milk: p21, p27, phospho-Chk1, Chk1, phospho-Chk2, Chk2, ZEB1, ZEB2, p53, and γH2AX (table S10).
Phospho chk2 antibody
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WebPhospho-Chk2 (Ser33/35) Antibody Cell Signaling Technology Polyclonal Antibody for studying Chk2 (Ser33/Ser35) phosphate. Cited in 26 publications. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Chk2 (Ser33/35) Antibody (CST #2665) is ready to ship. Go to your regional site? WebHuman Phospho-Chk2 (T68) Antibody Summary. Species Reactivity. Human. Specificity. Detects human Chk2 when phosphorylated at T68 in direct ELISAs and Western blots. …
WebThe Phospho-Chk1/2 Antibody Sampler Kit offers an economical means to evaluate the phosphorylation status of Chk1 and Chk2 on multiple residues. The kit contains enough … WebChk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven …
WebChk2 is a cell cycle checkpoint regulator and tumor suppressor. Along with Chk1, it plays a critical role in the cellular response to DNA damage, and is particularly important during … WebMar 25, 2024 · Flow cytometric analysis of phospho-Chk2 pThr68 on untreated (blue histogram) or UV-treated (green histogram) HeLa cells. Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a 1:800 dilution for 1 hour at room …
WebProduct name Anti-Phospho Serine/Threonine Chk1/Chk2 substrate antibody Description Rabbit polyclonal to Phospho Serine/Threonine Chk1/Chk2 substrate Host species Rabbit …
WebKey features and details Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply APC Rabbit monoclonal [Chk2T68-D12] to Chk2 (phospho T68) Suitable for: Flow Cyt Reacts with: Human Conjugation: APC. Ex: 645nm, Em: 660nm Related conjugates and formulations FITC PE Unconjugated simply smart big button combo-handbuchWebJul 18, 2024 · CHK2 is phosphorylated at tyrosine 156 in nocodazole-arrested M phase cells To determine whether there is any M phase-specific CHK2 phosphorylation, ectopically expressed CHK2 was... simply smart bloorWebPVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). ray voss obituaryWebPVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under … simply smart bottle couponWebAll lanes : Anti-Chk2 (phospho T68) antibody (ab226984) at 1/500 dilution Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract Lane 2 : Chk2 … rayvow fachinformationWebWestern blot - Phospho-Chk2-T68 Rabbit pAb (AP0590) Western blot analysis of extracts of HeLa cells, using Phospho-Chk2-T68 antibody (AP0590). HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. simply smart bramptonWebPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. Antibodies are purified by protein A and peptide affinity chromatography. Validations None provided Applications Immunoprecipitation (IP) Reactivity Chlorocebus sabaeus (Green monkey) rayvon williams watsonville