WebJan 31, 2024 · A recent structural study of a ternary complex between the MTase (in the context of the full-length NS5 protein), the partially capped RNA (at the N7 position) and S-adenosyl-L-homocysteine (the by-product of the reaction) allowed the authors to propose a detailed mechanism for 2’-O-ribose methylation of genomic viral RNA : The catalytic ... WebRNA modification plays an important role in modulating host-pathogen interaction. Flavivirus NS5 protein encodes N-7 and 29-O methyltransferase activities that are …
Frontiers Methyltransferase-Deficient Avian Flaviviruses Are ...
WebAug 28, 2010 · Despite the existence of two distinct methylation activities, the crystal structures of flavivirus MTases showed a single binding site for S-adenosyl-L … WebApr 25, 2024 · The genus Flavivirus encompasses several worldwide-distributed arthropod-borne viruses including, dengue virus, Japanese encephalitis virus, West Nile virus, … tlc interpreting services
Flavivirus RNA cap methyltransferase: structure, …
WebJan 18, 2024 · Over the last years, RNA methylation has emerged as a powerful mechanism to regulate messenger RNA metabolism at the posttranscriptional level. Not … WebAug 28, 2010 · Despite the existence of two distinct methylation activities, the crystal structures of flavivirus MTases showed a single binding site for S-adenosyl-L-methionine (SAM), the methyl donor. This finding indicates that the substrate GpppA-RNA must be repositioned to accept the N7 and 2′-O methyl groups from SAM during the sequential … WebJun 27, 2024 · The positive-sense flavivirus RNA genome bears a cap 1 structure essential for RNA stability and viral protein translation, and the formation of cap 1 requires the virally encoded nonstructural protein NS5 harboring guanylyltransferase (GTase), cap guanine N7 methyltransferase (N7 MTase), and 5'-nucleotide ribose 2'-O MTase activities in its … tlc interview 1999