Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. • Cy3 and Cy5 are the most popular, used typically combined for 2 colors detection. Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while … WebOur studies show that the formal C2H2excision from Cy5 occurs mainly through an intermolecular pathway involving a combination of bond cleavage and reconstitution while unambiguously confirming the identity of the fluorescent photoproduct of Cy5 to be Cy3 using various spectroscopic tools.

Cy5.5 Dye - an overview ScienceDirect Topics

Web• 100 nmoles Cy5-17-UTP, in 10 mM phosphate buffer, pH 7.0 5 mM concentration. Figure 2. Cy5 fluorescent dye absorption and fluorescence spectra Cy5-UTP characteristics the dark. Absorbance max 2.1.649 nm Extinction max 250 000 M-1cm-1 Emission max 670 nm Quantum yield >0.28 Additional reagents required WebCy5 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This far-red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. broadway consulting group

Cy5 dye Thermo Fisher Scientific - US

WebCy 5.5: Used as a fluorescent label in 5' nuclease probes for qPCR, Scorpion ® primers (Premier Biosoft), and Molecular Beacons. Cy 5.5 can also be used to label DNA oligos for use as hybridization probes in other applications, such as fluorescent in situ hybridization (FISH). Freedom rhodamine dyes WebMar 1, 2016 · Many fluorescent dyes can be used as markers. The cyanine fluorescent dye (Cy5 and Cy3) is composed of two indole rings and five or three methine groups. It can bind to nucleic acids and proteins in the ground state and its application is very wide. WebPhotostable, red emitting fluorescent dye with a free amino group for reaction with activated esters and activated carboxy groups. Analog of Cy5® amine. Cyanine5 amine, … broadway contractors